Overestimation of Incidence of Hepatitis B Virus Mixed-Genotype Infections by Use of the New Line Probe INNO-LiPA Genotyping Assay
نویسندگان
چکیده
منابع مشابه
Comparison of DNA enzyme immunoassay and line probe assays (Inno-LiPA HCV I and II) for hepatitis C virus genotyping.
Two methods for genotyping hepatitis C virus (DNA enzyme immunoassay [DEIA] and line probe assay [Inno-LiPA HCV I and II]) were compared on 120 samples and of these 87% were assigned to the same subtype by both assays. There were 15 subtyping discrepancies which involved 5% of type 1 isolates and 90% of type 2 isolates. Amplified products from the core and 5' untranslated regions (UTR) were seq...
متن کاملImprovement of hepatitis C virus (HCV) genotype determination with the new version of the INNO-LiPA HCV assay.
Hepatitis C virus (HCV) isolates have been classified into six main genotypes. Genotyping methods, and especially the widely used line probe assay (LiPA), are frequently based on the 5'-untranslated region (5'UTR). However, this region is not appropriate for discriminating HCV strains at the subtype level and for distinguishing many genotype 6 samples from genotype 1. We investigated the capaci...
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Genotypes A and D of the hepatitis B virus were found to be prevalent among 278 chronic carriers residing in Spain, and genotypes B, C, E and F were detected with significant frequency (9%). Two genotype E infections corresponded to carriers born in Spain who had never traveled to Africa. These results indicate that genotype E is beginning to circulate in the Spanish population in the same way ...
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Hepatitis C virus (HCV) genotyping is a tool used to optimize antiviral treatment regimens. The newly developed Versant HCV genotype assay (LiPA) 2.0 uses sequence information from both the 5' untranslated region and the core region, allowing distinction between HCV genotype 1 and subtypes c to l of genotype 6 and between subtypes a and b of genotype 1. HCV-positive samples were genotyped manua...
متن کاملRevision of interpretation criteria of the INNO-LiPA HBV genotyping assay.
We read with great interest the recent article published by Mercier et al. (4) on the overestimation of mixed infections as a result of erroneous genotype H detection by use of the new version of the INNO-LiPA HBV genotyping assay. In this study, the INNO-LiPA assay identified 28 mixed infections in a population of 200 HBsAgand anti-HBcAg-hepatitis B virus (HBV)-DNA-positive French blood donors...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2010
ISSN: 0095-1137
DOI: 10.1128/jcm.01888-10