Outer-membrane antigen expression by Moraxella (Branhamella) catarrhalis influences pulmonary clearance

Purification and characterization of a high-molecular-weight outer membrane protein of Moraxella (Branhamella) catarrhalis.

Moraxella (Branhamella) catarrhalis is an important bacterial cause of otitis media in children and lower respiratory tract infections in adults. In this study, we describe the presence of a novel high-molecular-weight outer membrane protein (HMW-OMP). This protein varies from 350 to 720 kDa in apparent molecular mass among strains by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (S...

Molecular cloning and characterization of outer membrane protein E of Moraxella (Branhamella) catarrhalis.

Outer membrane protein E (OMP E) is a 50-kDa protein of Moraxella (Branhamella) catarrhalis. It is a potential vaccine antigen because it is expressed on the surface of the bacterium and has antigenic determinants which are conserved among most strains of M. catarrhalis. To clone the gene encoding OMP E, an EMBL-3 genomic library of strain 25240 was screened with a family of degenerate oligonuc...

Phage antibodies obtained by competitive selection on complement-resistant Moraxella (Branhamella) catarrhalis recognize the high-molecular-weight outer membrane protein.

We used competitive panning to select a panel of 10 different human antibodies from a large semisynthetic phage display library that distinguish between serum complement-resistant and complement-sensitive strains of the gram-negative diplococcus Moraxella (Branhamella) catarrhalis. Western blotting analyses and inhibition enzyme-linked immunosorbent assays showed that all phage antibodies were ...

The outer membrane protein OlpA contributes to Moraxella catarrhalis serum

Moraxella (Branhamella) catarrhalis bacteremia in an immunocompetent children

Moraxella catarrhalis is an aerobic, Gram-negative diplococcus that commonly inhabits the upper respiratory tract (1). M. catarrhalis is now considered as an important cause of respiratory tract infections in children and adults with chronic obstructive pulmonary disease (COPD) (2, 3, 4). M. catarrhalis is now considered a common cause of otitis media in children and sinusitis in both children ...