Osteoclasts culture in compartmantalized microfluidic platforms and bone-like mineralized substrates
نویسندگان
چکیده
منابع مشابه
Micro-patterned Polystyrene Substrates for Highly Integrated Microfluidic Cell Culture
Adherent mammalian cells dynamically interact with their extracellular matrix (ECM) and culture substrate. To accommodate this sensitivity, standard culture techniques typically utilize tissue culture polystyrene (TCPS), a treated polystyrene substrate that promotes cell attachment. However, TCPS cannot be easily integrated into microfluidic devices as it is incompatible with conventional fabri...
متن کاملMicrofluidic platforms for mechanobiology.
Mechanotransduction has been a topic of considerable interest since early studies demonstrated a link between mechanical force and biological response. Until recently, studies of fundamental phenomena were based either on in vivo experiments with limited control or direct access, or on large-scale in vitro studies lacking many of the potentially important physiological factors. With the advent ...
متن کاملIn Vivo Ectopic Bone Formation by Devitalized Mineralized Stem Cell Carriers Produced Under Mineralizing Culture Condition
Functionalization of tissue engineering scaffolds with in vitro-generated bone-like extracellular matrix (ECM) represents an effective biomimetic approach to promote osteogenic differentiation of stem cells in vitro. However, the bone-forming capacity of these constructs (seeded with or without cells) is so far not apparent. In this study, we aimed at developing a mineralizing culture condition...
متن کاملEnhancement of static incubation time in microfluidic cell culture platforms exploiting extended air-liquid interface.
Microfluidics based cell culture applications have facilitated the study of cellular dynamics at the single entity level. Yet, long term versions of such applications in a static framework suffer from the fast exhaustion of available oxygen, dissolved in the limited media volume available per cell, within the microconfined environment. In order to circumvent such drawbacks, we have improvised a...
متن کاملToward culture of single gametes: the development of microfluidic platforms for assisted reproduction.
During the last few decades in vitro production of mammalian embryos and assisted reproductive technologies such as embryo transfer, cryopreservation, and cloning have been used to produce and propagate genetically superior livestock. However, efficiencies of these technologies remain low. For these technologies to become more commercially viable, the efficiencies must improve. Despite this imp...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Frontiers in Bioengineering and Biotechnology
سال: 2016
ISSN: 2296-4185
DOI: 10.3389/conf.fbioe.2016.01.00612