Osteoblast response (initial adhesion and alkaline phosphatase activity) following exposure to a barrier membrane/enamel matrix derivative combination
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چکیده
منابع مشابه
Effect f Enamel Matrix Derivative n Alkaline Phosphatase Activity n Osteoblast Cells Through he Mitogen-activated Protein Kinase Pathway
The study examined the response of MG63 to Enamel Matrix Derivative (EMD) on the coarse-grit-blasted and acid-etched surface disks. We characterized associated changes in proliferation and differentiation related intracelluar signaling molecular including mitogen activitied protein kibase. The major groups of MAPKs (ERK1/2, cJUN-terminal kinases, JNK and p38-MAPK) have been characterized in pre...
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Tissue-nonspecific alkaline phosphatase (TNAP) is essential for bone matrix mineralization, but the central mechanism for TNAP action remains undefined. We observed that ATP-dependent (45)Ca precipitation was decreased in calvarial osteoblast matrix vesicle (MV) fractions from TNAP-/- mice, a model of infantile hypophosphatasia. Because TNAP hydrolyzes the mineralization inhibitor inorganic pyr...
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Introduction: Hydatid cyst disease is caused by the protoscolices of Echinococcus granulosus. Alkaline phosphatase (ALP) enzyme is required for metabolism, physiology, immunology, and nutrients absorption in parasite. The aim of this study was to compare the level of ALP activity (as a pathological biomarker) in hydatid cyst protoscolices (HCP) with that of sheep liver tissue and to determine t...
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OBJECTIVE The aim of this study was to investigate the effects of PRP on SAOS-2 cells in terms of cytokine expression, cell activity and oxidative stress. DESIGN Cell line SAOS-2 (1×10(5)cells/mL) were grown in culture medium α-MEM with 10% FBS for 24h and stimulated (or not) with PRP at concentrations of 3, 10 and 20%, LPS (E. coli, 10g/mL) and IL-1β (1mg/mL) for 24h. The supernatant was col...
متن کاملOsteoblast Differentiation on Collagen Scaffold with Immobilized Alkaline Phosphatase
Background In tissue engineering, scaffold characteristics play an important role in the biological interactions between cells and the scaffold. Cell adhesion, proliferation, and activation depend on material properties used for the fabrication of scaffolds. Objective In the present investigation, we used collagen with proper characteristics including mechanically stability, biodegradability ...
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ژورنال
عنوان ژورنال: Indian Journal of Dental Research
سال: 2009
ISSN: 0970-9290
DOI: 10.4103/0970-9290.49048