Osmoregulation in symbiosis-independent mutants of Bdellovibrio bacteriovorus
نویسندگان
چکیده
منابع مشابه
Osmoregulation in symbiosis-independent mutants of Bdellovibrio bacteriovorus.
Bdellovibrios capable of axenic growth grow in a cell-free medium at a rate considerably lower than that attainable in a two-membered culture with Escherichia coli. The axenic growth rate may be improved either by adjustment of the osmosity of the medium or by the addition of low concentrations of spermine.
متن کاملSymbiosis-independent and symbiosis-incompetent mutants of Bdellovibrio bacteriovorus 109J.
Symbiosis-independent (Sin) mutants were isolated from the symbiosis-dependent and symbiosis-competent (Sdcomp+) Bdellovibrio bacteriovorus 109J. Independently isolated Sin mutants were examined for their symbiosis competence and most were found to be comp+. Bdellovibrios comp- were selected from the Sincomp+ mutants. The Sincomp+ bdellovibrios are always at a selective disadvantage, either aga...
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A variety of temperature-sensitive mutants of host-dependent Bdellovibrio bacteriovorus 109D were selected after ethyl methane sulfonate mutagenesis. Mutants that demonstrated plaque-forming ability reversion frequencies of 10(-8) to 10(-9) were chosen for further study. Representatives of these mutants were then characterized by phase-contrast and electron microscopy, temperature-shifted one-s...
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Bdellovibrio bacteriovorus was capable of parasitizing Shigella boydii, S. flexneri, and S. sonnei. The bdellovibrio was able to produce plaques on lawns consisting of heat-killed or ultraviolet-irradiated S. boydii.
متن کاملDevelopment of a novel genetic system to create markerless deletion mutants of Bdellovibrio bacteriovorus.
Bdellovibrio bacteriovorus is a species of unique obligate predatory bacteria that utilize gram-negative bacteria as prey. Their life cycle alternates between a motile extracellular phase and a growth phase within the prey cell periplasm. The mechanism of prey cell invasion and the genetic networks and regulation during the life cycle have not been elucidated. The obligate predatory nature of t...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1977
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.33.5.1207-1208.1977