Ornithine Carbamoyltransferase from Halobacterium salinarium
نویسندگان
چکیده
منابع مشابه
Ornithine carbamoyltransferase deficiency.
The death of Jesse Gelsinger on 17 September 1999 had major eVects on the gene therapy community. It brought to a halt a gene therapy clinical trial at the Institute of Human Gene Therapy, University of Pennsylvania, USA and brought to a wider audience the potential clinical problems associated with this technology. In addition a number of clinicians became aware of Jesse’s genetic disorder, or...
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Arginine deiminase from the extreme halophilic archaebacterium Halobacterium salinarium was purified to homogeneity in a four-step procedure with a 310-fold enrichment. The enzyme consists of two identical subunits of 55 kDa; its native molecular mass is 105 kDa. The pI of 4.7 indicates that acidic nature of the protein, which is evidenced by its amino acid composition, which shows an excess of...
متن کاملCatabolic ornithine transcarbamylase of Halobacterium halobium (salinarium): purification, characterization, sequence determination, and evolution.
Halobacterium halobium (salinarium) is able to grow fermentatively via the arginine deiminase pathway, which is mediated by three enzymes and one membrane-bound arginine-ornithine antiporter. One of the enzymes, catabolic ornithine transcarbamylase (cOTCase), was purified from fermentatively grown cultures by gel filtration and ammonium sulfate-mediated hydrophobic chromatography. It consists o...
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Ornithine carbamoyltransferase has been purified from the liver of the loggerhead turtle Caretta caretta by a single-step procedure using chromatography on an affinity column to which the transition-state analogue, delta-N-(phosphonoacetyl)-L-ornithine (delta-PALO), was covalently bound. The procedure employed yielded an enzyme which was purified 373-fold and was judged to be homogeneous by non...
متن کاملHalophilic enzymes in cell-free extracts of Halobacterium salinarium.
Cells of the obligately halophilic bacterium Halobacterium salinarium have been shown to contain high concentrations of sodium and potassium chloride (Christian and Ingram, J. Gen. Microbiol. 20:27, 1959; Christian and Waltho, Biochim. Biophys. Acta 65:506, 1962). Enzymes extracted from this organism require up to 25% NaCl for optimal activity (Baxter and Gibbons, Can. J. Microbiol. 2:599, 1956...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1972
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1972.tb01847.x