Organic and aqueous extraction of lipids from birch pollen grains exposed to gaseous pollutants

The lipid fraction of birch pollen grains (BPGs) is not yet fully described, although molecules may play a role in the allergic immune response. mechanisms by which atmospheric pollutants modify allergenic (PGs) are also far from being elucidated despite high potential effects on sensitization. This work contribution to better description profile (both external and cytoplasmic) BPGs alterations induced gaseous air pollutants. Several extractions were performed using organic aqueous solvents following exposure ozone and/or nitrogen dioxide under conditions favoring release internal lipids. Ozone reacted with alkenes produce aldehydes saturated fatty acids, while was shown be unreactive NO2 exhibited protective effect against reactivity ozone, probably competition for adsorption sites. decreased during simultaneous NO2/O3 raised possibility Langmuir-Hinshelwood mechanism. Oxidation reactions did substantially extraction lipids solvent, suggesting that bioaccessibility modified oxidation. On contrary, rupture PGs appeared key factor enhancing bioactive mediators (linoleic α-linolenic acids) an solution. has specific characteristics compared lipids, more abundant hexadecanoic acid, tricosanol, particularly unsaturated acids acids). action identified this study: can (i) alkenes, (ii) adsorb surface source oxidative stress after inhalation PGs, (iii) promote cytoplasmic facilitating rupture.