Oral Squamous Cells and Age Estimation in Exfoliative Cytology with Hematoxylin and Eosin Stain– A Quantitative Study

Introduction: Exfoliative cytology in age estimation is a simple, painless, less invasive collection of exfoliative cells from epithelial layers, used as diagnostic aid for estimation. The oral cavity an ideal site with physiological turnover cells, decreases the increases show variation cellular morphological changes. Age one important factors to identify individual and also helps know chronological person.
 Aim: To analyze estimate buccal smear comparing average size under Image morphometric analysis.
 Materials Methods: Buccal mucosal smears are taken using wooden spatula gentle motion scraping smeared on clean glass slide fixed 95% ethanol immediately after smearing minimum around 15 minutes stained Haematoxylin eosin stain. After staining, were observed by microscope measured paint tool. Pearson correlation analysis was done SPSS software.
 Results: cell nuclear difference values coefficient statistically significant p value<0.05 revealing that there shrinkage increase age.
 Conclusion: successful vastly growing technology detection premalignant lesions.