Optimized Sample Preparation for Tandem Capillary Electrophoresis Single-Stranded Conformational Polymorphism/Heteroduplex Analysis
نویسندگان
چکیده
منابع مشابه
Introducing a New and Simple Protocol for Capillary Electrophoresis of Cell Free Fetal Double Stranded DNA
Isolation of cell free fetal DNA (cffDNA) from maternal serum usually leads to very low concentrations of DNA impeding further resolving through conventional methods of electrophoresis. Although several protocols have been described for capillary electrophoresis (CE) of double stranded DNA, they usually need using special polymers or coated capillaries which degrade over time. Herein, we propos...
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We developed a novel single-step capillary electrophoresis (SSCE) scheme for field-amplified sample stacking (FASS) by using a hydrophilic material polymethyl methacrylate (PMMA) microchannel chip. The capillary effect was used to introduce liquids and control capillary stop valves in a partial barrier structure of the microchannel. Through the combined action of stop valves and air vents, both...
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A simple and rapid stability indicating method based on capillary zone electrophoresis has been developed and validated for the analysis of buserelin (BUS). The best separations were achieved by using a bare fused silica capillary (75 m i.d.; 65.5 cm total, 57.0 cm effective length), phosphate buffer (pH = 3.00; 26.4 mM), at 35 °C. The sample was hydrodynamically injected at 50 mbar for 5 seco...
متن کاملA stability indicating capillary electrophoresis method for analysis of buserelin
A simple and rapid stability indicating method based on capillary zone electrophoresis has been developed and validated for the analysis of buserelin (BUS). The best separations were achieved by using a bare fused silica capillary (75 m i.d.; 65.5 cm total, 57.0 cm effective length), phosphate buffer (pH = 3.00; 26.4 mM), at 35 °C. The sample was hydrodynamically injected at 50 mbar for 5 seco...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2002
ISSN: 0736-6205,1940-9818
DOI: 10.2144/02332st04