Optimization of Ultrasonic-assisted Extraction of Total Phenolic Compound from Leaves of Chromolaena Odorata L. Using Response Surface Methodology

Chromolaena odorata L. or C. is a weed that found in tropical continents . Leaves, roots and flowers of have been used as medicinal plants for centuries. Preliminary phytochemical screening showed the chemical composition its extracts having phenolic compounds such flavonoids, saponins, tannins steroids. The total (TPC) are acknowledged biochemical activities antiinflammatory, antiviral, antimicrobial, antioxidant agents etc. This work interested antimicrobial activity an ethanolic extract leaves. Though, many solvents can be to TPC from plants. Ethanol with ultrasound assisted extraction was selected. response surface methodology coupled nonlinear solver were find optimal variables. Central Composite Design (CCD) employed sampling technique. Three variables ratio ethanol dried leaves (X 1 ), aqueous concentration 2 time 3 ). conditions fixed at 40 kHz 60 ˚C. maximum yield 111.77 mg GAE/g dry 57 % v/v concentration, 43 mL solvent/g sample 35 minutes time.