Optimization of the autoclave-assisted alkaline delignification of cocoa (Theobroma cacao) pod husks using KOH to maximize reducing sugars

Cocoa pod husks are a type of biomass that is still poorly explored. This work was carried out as part the optimization delignification process for this residue with potassium hydroxide, to maximize reducing sugars content. Screening potentially influencing factors showed solvent ratio and temperature had greatest effect on process. Optimization these using composite central plan revealed quadratic model most suitable predicting rate delignification. The predicted R² (0.815) in good agreement adjusted (0.906). correlation coefficient (R2 = 0.945) between experimental results confirmed fit model. optimal conditions were 9.14% 128 °C, which resulted maximum degree 93.9%, 80% solids recovered. study found removal extractables before pretreatment considerably improved cocoa production sugars, increased from 3.15 ± 0.006 mg/mL 5.33 0.143 mg/mL. Scanning electron microscopy X-ray diffraction physicochemical changes after pretreatment.