Optimization of random amplified polymorphic DNA protocol for molecular identification of Lophius gastrophysus

Molecular Characterization of Iraqi Local Goat Breeds Using Random Amplified Polymorphic DNA Markers

Cashmere is only produced from Maraz (Kurdi) goat breed in Iraqi Kurdistan Region. The objective of this study was to assess the genetic diversity of different Maraz color types, black goat and Shami breeds using random amplified polymorphic DNA (RAPD) markers. Fourteen primers were used and nine out of them were selected based on their number of bands (NB) and polymorphic characteristics. Thes...

Identification of Leishmania Species Causing Cutaneous Leishmaniasis Using Random Amplified Polymorphic DNA (RAPD-PCR) in Kharve, Iran

Background: Leishmaniasis, especially cutaneous leishmaniasis, is considered an important health problem in many parts of Iran including Kharve, Khorasan Razavi province. Cutaneous leishmaniasis is caused by various species of Leishmania, each having a different secondary host. Thus, identifying the parasites’ specie is of paramount importance for containment strategy planning. The morpho...

Random amplified polymorphic DNA (RAPD) fingerprinting of Mycoplasma gallisepticum isolates from chickens

  Ten field isolates of Mycoplasma gallisepticum (MG) from different geographical areas of Iran were analyzed by random amplification of polymorphic DNA (RAPD). RAPD analysis produced reproducible banding patterns on the basis of which various distinct amplification patterns could be detected. MG isolates compared with reference strains (S6 and Mg SS) and vaccine strain (ts-11) and demonstrated...

Molecular Identification of PCR and TaqMan Automated Detection in Utility of Random Amplified Polymorphic DNA

identification of leishmania species isolated from human cutaneous leishmaniasis, using random amplified polymorphic dna (rapd-pcr)

this cross-sectional study was designed to isolate of leishmania spp from cutaneous leishmaniasis patients and characterized them by rapd-pcr technique. eighty- seven leishmania isolates from 112 samples were collected from cutaneous leishmaniasis (cl) patients who referred to mashhad health centers from august 2002 to may 2004. desirable samples (87 isolates) were characterized by rapd-pcr met...