Optimization of Pulse-Field Gel Electrophoresis for Subtyping of Klebsiella pneumoniae
نویسندگان
چکیده
منابع مشابه
Optimization of Pulse-Field Gel Electrophoresis for Subtyping of Klebsiella pneumoniae
A total of 110 strains of Klebsiella pneumoniae were used to optimize pulsed-field gel electrophoresis (PFGE) for subtyping of K. pneumoniae. For optimization of electrophoresis parameters (EPs) of XbaI-PFGE, 11 isolates were analyzed with XbaI digestion using three EPs. The EP of a switch time of 6 to 36 s for 18.5 h gave clearest patterns and was declared the optimal EP for XbaI PFGE of K. pn...
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ژورنال
عنوان ژورنال: International Journal of Environmental Research and Public Health
سال: 2013
ISSN: 1660-4601
DOI: 10.3390/ijerph10072720