Optimization of mRNA untranslated regions for improved expression of therapeutic mRNA
نویسندگان
چکیده
منابع مشابه
Influence of untranslated regions on retroviral mRNA transfer and expression
BACKGROUND Deliberate cellular reprogramming is becoming a realistic objective in the clinic. While the origin of the target cells is critical, delivery of bioactive molecules to trigger a shift in cell-fate remains the major hurdle. To date, several strategies based either on non-integrative vectors, protein transfer or mRNA delivery have been investigated. In a recent study, a unique modifica...
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Balancing the amounts of enzymes is one of the important factors to achieve optimum performance of a designed metabolic pathway. However, the random mutagenesis approach is impractical since it requires searching an unnecessarily large number of variants and often results in searching a narrow range of expression levels which are out of optimal level. Here, we developed a predictive combinatori...
متن کاملSystematic genomic screening and analysis of mRNA in untranslated regions and mRNA precursors: combining experimental and computational approaches
Motivation: The untranslated regions (UTRs) of mRNA upstream (5′UTR) and downstream (3′UTR) of the open reading frame, as well as the mRNA precursor, carry important regulatory sequences. To reveal unidentified regulatory signals, we combine information from experiments with computational approaches. Depending on available knowledge, three different strategies are employed. Results: Searching w...
متن کاملSystematic genomic screening and analysis of mRNA in untranslated regions and mRNA precursors: combining experimental and computational approaches
MOTIVATION The untranslated regions (UTRs) of mRNA upstream (5'UTR) and downstream (3'UTR) of the open reading frame, as well as the mRNA precursor, carry important regulatory sequences. To reveal unidentified regulatory signals, we combine information from experiments with computational approaches. Depending on available knowledge, three different strategies are employed. RESULTS Searching w...
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ژورنال
عنوان ژورنال: RNA Biology
سال: 2018
ISSN: 1547-6286,1555-8584
DOI: 10.1080/15476286.2018.1450054