Optimization of high-density cDNA-microarray protocols by 'design of experiments'

Optimization of high-density cDNA-microarray protocols by 'design of experiments'.

Expression analysis using microarray technology implies a complex experimental procedure with a large number of parameters affecting the final result. We have demonstrated that optimization of such a complex protocol can be far better handled using design of experiments (DOE) than by working on a single parameter at a time. Based on the results of a screening design, we developed a spotting buf...

cDNA Microarray Data Normalization

Spatial Analysis of cDNA Microarray Experiments

Some normalization methods assume that the two channels of intensities are related by a constant so that Microarray experiments allow RNA level measurements for many the center of the distribution of the log of the ratio is genes in multiple samples. However, mining the biological information shifted to zero. Chen et al. (1997) proposed an iterative from the large sets of data generated by micr...

Segmentation of cDNA microarray images by kernel density estimation

The segmentation of cDNA microarray spots is essential in analyzing the intensities of microarray images for biological and medical investigation. In this work, nonparametric methods using kernel density estimation are applied to segment two-channel cDNA microarray images. This approach groups pixels into both a foreground and a background. The segmentation performance of this model is tested a...

an investigation into translation of cultural concepts by beginner and advance student using think – aloud protocols

this research aims at answering the questions about translation problems and strategies applied by translators when translating cultural concepts. in order to address this issue, qualitative and quantitative study were conducted on two groups of subjects at imam reza international university of mashhad. these two groups were assigned as beginner and advanced translation students (10 students). ...