Optimization of gamma-Aminobutyric Acid Bioconversion by Recombinant Escherichia coli
نویسندگان
چکیده
منابع مشابه
Effect of protein and lactulose on the production of gamma-aminobutyric acid by faecal Escherichia coli.
The value of lactulose treatment in hepatic encephalopathy is widely recognised but its mode of action remains controversial. Much evidence supports a role for gamma-aminobutyric acid in hepatic encephalopathy, and lactulose could alter its bacterial production in the gut. Using the rat synaptic membrane assay and gas chromatography mass spectrometry, the production of gamma-aminobutyric acid b...
متن کاملEnhanced Expression of Recombinant Activin A in Escherichia coli by Optimization of Induction Parameters
Activin A is a member of the transforming growth factor β super family. Because of its extensive clinical usages, its recombinant production is beneficial. In this study, activin A was expressed in E. coli using the pET 21a expression vector. The optimization of the activin A production in E. coli was done by using the response surface methodology (RSM). At this stage, the effect of IPTG and la...
متن کاملBioconversion of pinoresinol into matairesinol by use of recombinant Escherichia coli.
Lignans, a class of dimeric phenylpropanoid derivative found in plants, such as whole grains and sesame and flax seeds, have anticancer activity and can act as phytoestrogens. The lignans secoisolariciresinol and matairesinol can be converted in the mammalian proximal colon into enterolactone and enterodiol, respectively, which reduce the risk of breast and colon cancer. To establish an efficie...
متن کاملdetermination of gamma - aminobutyric acid by high-performance liquid chromatography
gamma-aminobutyric acid is the main inhibitory amino acid neurotransmitter in several areas of the brain. measurement of gamma-aminobutyric acid in the brain will help elucidate its metabolic rote and diagnostic value. the liquid chromatography (lc) system consisted of a delivery pump. male rats (150-250g) were used in this study. the brain was rapidly removed from decapitated animals. trie chr...
متن کاملOptimization of Cloning Conditions for high-level Production of Recombinant Mouse Interleukin-2 in Escherichia coli
Backgrounds and objectives: Interleukin 2 (IL-2) secreted by activated CD4+ T cells has been known as a major mediator in both adaptive and native immune system due to a board range of effects on different cells in the immunity system (1-6). Methods: cDNA synthesis was performed using gene- specific primers designed by Gene Runner software after RNA extraction of mouse splenocytes. PCR pro...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: KSBB Journal
سال: 2012
ISSN: 1225-7117
DOI: 10.7841/ksbbj.2012.27.2.127