Optimization of Cellulase-Assisted Extraction of Total Flavonoids from Equisetum via Response Surface Methodology Based on Antioxidant Activity
نویسندگان
چکیده
Flavonoids are one of the most essential compounds in various plants. This study used cellulase-assisted method to extract flavonoid-enriched antioxidants from Equisetum arvense L. (EAL). According a three-factor and three-level central composite design, response surface methodology was obtain maximum total flavonoid yield, thereby providing potent antioxidant activity EAL extracts. The results indicated that optimal condition for extraction 0.52% (base substrate) enzyme concentration enzymolysis 50.58 min at 49.03 °C. rate flavonoids horsetail reached 4.88 mg/g under these conditions. yield 51.23% higher than traditional solid–liquid method. highest DPPH-scavenging ability extracts obtained 0.50% 49.41 46.59 °C 77.36%. HPLC revealed substances had diverse flavonoids. Thus, consistent Therefore, current provided an efficient EAL, facilitating technical support utilizing plant.
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ژورنال
عنوان ژورنال: Processes
سال: 2023
ISSN: ['2227-9717']
DOI: https://doi.org/10.3390/pr11071978