Optimization of Agrobacterium-mediated transformation procedure for an indica rice variety-Khang Dan 18

In addition to traditional methods, advanced biotechnologies, especially CRISPR/Cas9-mediated genome editing, have emerged as effective tools for improving important agronomic traits in rice. However, the critical step utilizing these systems is develope an system rice transformation and regeneration. This study was performed establish procedures Agrobacterium-mediated regeneration of cultivar Khang Dan 18 (KD18) – a popular indica variety North Vietnam. The tissue cultures procedure with optimized medium compositions showed high frequencies callus induction shoot regeneration, at 94.7% 45.3% respectively. We found that 30 mg/L hygromycin concentration transgenic KD18 selection. Light-yellow friable calli were used starting material mediated by A. tumefaciens strain AGL1 harbouring pHUE411 vector containing gus intron resistance gene (hptII). Important factors related had been this study. frequency (12.8%) achieved using cultivar. which, bacterial density (OD600), infection time co-cultivation period 0.1, 20 minutes 3 days, PCR analysis specific primers histological GUS staining demonstrated presence hptII genes lines. result provides potential protocol transfer interest into well other cultivars.