Optimization of a Quantitative Micro-neutralization Assay

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Quantitative neutralization assay of fungicidal activity of disinfectants.

A quantitative assay using a neutralization medium was developed to evaluate fungicidal activity of disinfectants. Concentrated Dey-Engley neutralizing broth was used in this study and was demonstrated to inactivate various chemical agents within 5 min when disinfectant concentrations were reduced to specific levels. Addition of this Dey-Engley broth to test tubes containing fungal cells and di...

متن کامل

Quantitative Bacterial Micro-Assay for Rapid Diagnosis of Galactosemia: Application in Galactosemia Neonatal Screening

In the present study a new economic and rapid bacterial micro-assay for simultaneous detection and quantitative measurement of serum galactose was developed. Analysis of the standard curve showed a linearity range for galactose from 2 mg/dL to 180 mg/dL with a regression equation of Y = 0.013X ? 0.083; R² = 0.962. The advantage of the method is its ability to measure serum galactose quantitativ...

متن کامل

Putative neutralization epitopes and broad cross-genotype neutralization of Hepatitis E virus confirmed by a quantitative cell-culture assay.

Monolayers of Hep G2/C3A cells were inoculated with genotype 1 Hepatitis E virus (HEV) mixed with either anti-HEV or an appropriate control. After 5 or 6 days, cell monolayers were stained with anti-HEV and infected cells were identified by immunofluorescence microscopy and counted. Anti-HEV from vaccinated or infected rhesus monkeys neutralized the virus, as did mAbs that recognized epitopes o...

متن کامل

A Sensitive Neutralization Assay for Influenza C Viruses Based on the Acetylesterase Activity HEF Glycoprotein

Influenza C virus possesses specific neuraminate-O-acetylesterase as a receptor-destroying function. This enzymatic activity of the viral glycoprotein HEF (Hemagglutinin, esterase activity and fusion factor) can be visualized in situ by the use of distinct color substrates. Hereby the localization, as well as the quantity of synthesized HEF protein is detectable. We further developed the estera...

متن کامل

quantitative bacterial micro-assay for rapid diagnosis of galactosemia: application in galactosemia neonatal screening

in the present study a new economic and rapid bacterial micro-assay for simultaneous detection and quantitative measurement of serum galactose was developed. analysis of the standard curve showed a linearity range for galactose from 2 mg/dl to 180 mg/dl with a regression equation of y = 0.013x ? 0.083; r² = 0.962. the advantage of the method is its ability to measure serum galactose quantitativ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Journal of Visualized Experiments

سال: 2016

ISSN: 1940-087X

DOI: 10.3791/54897