Optimization of a Precolumn OPA Derivatization HPLC Assay for Monitoring of l-Asparagine Depletion in Serum during l-Asparaginase Therapy

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L-asparagine depletion and L-asparaginase activity in children with acute lymphoblastic leukemia receiving i.m. or i.v. Erwinia C. or E. coli L-asparaginase as first exposure.

PURPOSE The present study was aimed at investigating L-asparaginase (L-ASE) activity (in plasma) and L-asparagine (L-ASN) depletion (in plasma and CSF) in children with newly diagnosed acute lymphoblastic leukemia (ALL) exposed for the first time to different L-ASE products. PATIENTS AND METHODS During the induction treatment of the AIEOP ALL 95 study, 62 patients were treated with either Erw...

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Guinea pig serum L-asparaginase. Properties, purification, and application to determination of asparagine in biological samples.

After the first isolation of asparagine in 1806 by Vauquelin and Robiquet (l), there were numerous studies of its properties and physiological significance (2-4). Piria (5) in 1848 was probably the first to adduce evidence for a physiological role of asparagine during the life cycle of plants, and also to observe its transformation or metabolism by yeast. However, it was not until approximately...

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L-Asparagine synthetase in serum as a marker for neoplasia.

L-Asparagine synthetase appears in serum approximately 7 days after the s.c. implantation of 1 X 10(5) cells of Leukemia 5178Y/AR (resistant to L-asparaginase) and increases in activity as the neoplasm grows and metastasizes. The principal source of the enzyme is the primary tumor. After intravranial inoculation of tumor, the rate of leakage of the enzyme is more pronounced than when the subcut...

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Diabetes ketoacidosis in L-asparaginase therapy

Case E.C, 10 years old female brought to the ER due abdominal pain. Patient is a diagnosed case of B-Cell Acute Lymphoblastic Leukemia (ALL) thru clinical symptoms, laboratory work-ups and flow cytometry since 2 months prior to her present admission. She has been receiving 8 doses of L-asparaginase (leunase) and Prednisone (60mg/day for a week then 40mg/day for 3 weeks) for almost one month bef...

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ژورنال

عنوان ژورنال: Journal of Chromatographic Science

سال: 2018

ISSN: 0021-9665,1945-239X

DOI: 10.1093/chromsci/bmy053