Optimising synchrotron facilities for data collection in protein crystallography
نویسندگان
چکیده
منابع مشابه
Data Collection in Protein Crystallography: Capillary Effects and Background Corrections*
In protein crystallography, observed diffraction intensities must be corrected for background radiation due to scatter from air and scatter and absorption by capillary., crystal and mother liquor. A systematic study shows that a major contribution to background intensity is air scatter arising from the air intercepted by the direct X-ray beam as 'seen' by the receiving-counter aperture. As a re...
متن کاملDevelopment of a dose-limiting data collection strategy for serial synchrotron rotation crystallography
Serial crystallography, in which single-shot diffraction images are collected, has great potential for protein microcrystallography. Although serial femtosecond crystallography (SFX) has been successfully demonstrated, limited beam time prevents its routine use. Inspired by SFX, serial synchrotron crystallography (SSX) has been investigated at synchrotron macromolecular crystallography beamline...
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Here, an automated procedure is described to identify the positions of many cryocooled crystals mounted on the same sample holder, to rapidly predict and rank their relative diffraction strengths and to collect partial X-ray diffraction data sets from as many of the crystals as desired. Subsequent hierarchical cluster analysis then allows the best combination of partial data sets, optimizing th...
متن کاملMail-in data collection at SPring-8 protein crystallography beamlines
A mail-in data collection system makes it possible for beamline users to collect diffraction data without visiting a synchrotron facility. In the mail-in data collection system at SPring-8, users pack crystals into sample trays and send the trays to SPring-8 via a courier service as the first step. Next, the user specifies measurement conditions and checks the diffraction images via the Interne...
متن کاملStrategies for macromolecular synchrotron crystallography.
Introduction During the past ten years the rate of protein structure determination by X-ray crystallography has risen about tenfold [1]. The use of synchrotron radiation was instrumental for this growth and the fraction of the results obtained this way increased from 28% in 1990 to 80% in 1999 (Figure 1). There are 52 synchrotron stations worldwide used for macromolecular crystallography, and t...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography
سال: 1996
ISSN: 0108-7673
DOI: 10.1107/s0108767396098297