Optimisation of Recombinant Production of Active Human Cardiac SERCA2a ATPase
نویسندگان
چکیده
منابع مشابه
Optimisation of Recombinant Production of Active Human Cardiac SERCA2a ATPase
Methods for recombinant production of eukaryotic membrane proteins, yielding sufficient quantity and quality of protein for structural biology, remain a challenge. We describe here, expression and purification optimisation of the human SERCA2a cardiac isoform of Ca(2+) translocating ATPase, using Saccharomyces cerevisiae as the heterologous expression system of choice. Two different expression ...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2013
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0071842