Optimal LED Filtering for Fluorescence Microscopy
نویسندگان
چکیده
منابع مشابه
LED Fluorescence Microscopy for the Diagnosis of Pulmonary Tuberculosis: A Multi-Country Cross-Sectional Evaluation
BACKGROUND The diagnosis of tuberculosis (TB) in resource-limited settings relies on Ziehl-Neelsen (ZN) smear microscopy. LED fluorescence microscopy (LED-FM) has many potential advantages over ZN smear microscopy, but requires evaluation in the field. The aim of this study was to assess the sensitivity/specificity of LED-FM for the diagnosis of pulmonary TB and whether its performance varies w...
متن کاملLED-Fluorescence Microscopy for Diagnosis of Pulmonary Tuberculosis under Programmatic Conditions in India
BACKGROUND Light-emitting diode fluorescence microscopy (LED-FM) has been shown to be more sensitive than conventional bright field microscopy using Ziehl-Neelsen (ZN) stain in detecting sputum smear positive tuberculosis in controlled laboratory conditions. In 2012, Auramine O staining based LED-FM replaced conventional ZN microscopy in 200 designated microscopy centres (DMC) of medical colleg...
متن کاملEvaluation of the ParaLensTM LED microscope attachment versus standard fluorescence microscopy for detection of Mycobacteria
The ParalensTM (PL) microscope attachment converts a light microscope into an epi-fluorescence microscope. We compared the PL to standard fluorescence microscopy for detection of Mycobacteria in clinical and spiked samples. Overall agreement between the two systems was 100%. Quantitative and qualitative performance was comparable. The PL is an acceptable alternative to standard fluorescence mic...
متن کاملPerformance of Three LED-Based Fluorescence Microscopy Systems for Detection of Tuberculosis in Uganda
BACKGROUND Direct smear microscopy using Ziehl-Neelsen (ZN) staining is the mainstay of tuberculosis (TB) diagnosis in most high burden countries, but is limited by low sensitivity in routine practice, particularly in high human immunodeficiency virus (HIV) prevalence settings. METHODS We compared the performance of three commercial light emitting diode (LED)-based microscopy systems (Primost...
متن کاملPerformance of LED fluorescence microscopy for the detection of tuberculosis in Rwanda using Zeiss Primo Star
Introduction: Ziehl-Neelsen (ZN) bright-field microscopy is time-consuming, with poor sensitivity, even under optimal conditions. Introduction of Primo Star iLED fluorescent microscopy (FM) may improve TB case finding at referral hospitals in Rwanda. The study aimed to determine the acceptability and effectiveness of iLED in a low resource setting. Methods: Between June 2009 and May 2010, the R...
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ژورنال
عنوان ژورنال: Microscopy Today
سال: 2019
ISSN: 1551-9295,2150-3583
DOI: 10.1017/s155192951900083x