Opposite replication polarities of transcribed and nontranscribed histone H5 genes.
نویسندگان
چکیده
منابع مشابه
Opposite replication polarities of transcribed and nontranscribed histone H5 genes.
We used an in vitro nuclear runoff replication assay to analyze the direction of replication of the active and inactive histone H5 genes in avian cells. In embryonic erythrocytes the transcribed histone H5 gene displayed sensitivity to endogenous nuclease cleavage. In contrast, this gene was insensitive to endogenous nuclease digestion under the same conditions in nuclei of the lymphoblastoid c...
متن کاملReplication-dependent histone genes are actively transcribed in differentiating and aging retinal neurons.
In the mammalian genome, each histone family contains multiple replication-dependent paralogs, which are found in clusters where their transcription is thought to be coupled to the cell cycle. Here, we wanted to interrogate the transcriptional regulation of these paralogs during retinal development and aging. We employed deep sequencing, quantitative PCR, in situ hybridization (ISH), and microa...
متن کاملHistone H3 variants and modifications on transcribed genes.
C hromatin structure is disrupted by the process of gene transcription, and the state of the histones plays an important role in gene regulation. Transcribing genes tend to be marked by specific histone modifications that are thought to modulate transcriptional activity. Recently, Ahmad and Henikoff (1) found that transcribed genes in Drosophila are also enriched for histone variant H3.3, which...
متن کاملA Full Suite of Histone and Histone Modifying Genes Are Transcribed in the Dinoflagellate Lingulodinium
BACKGROUND Dinoflagellates typically lack histones and nucleosomes are not observed in DNA spreads. However, recent studies have shown the presence of core histone mRNA sequences scattered among different dinoflagellate species. To date, the presence of all components required for manufacturing and modifying nucleosomes in a single dinoflagellate species has not been confirmed. METHODOLOGY AN...
متن کاملNucleotide excision repair and photolyase preferentially repair the nontranscribed strand of RNA polymerase III-transcribed genes in Saccharomyces cerevisiae.
A high-resolution primer extension technique was used to study the relationships between repair, transcription, and mutagenesis in RNA polymerase III transcribed genes in Saccharomyces cerevisiae. The in vivo repair of UV-induced DNA damage by nucleotide excision repair (NER) and by photoreactivation is shown to be preferential for the nontranscribed strand (NTS) of the SNR6 gene. This is in co...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 1988
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.8.4.1657