Oomycete-specific ITS primers for identification and metabarcoding

Application of specific primers and polymerase chain reaction for identification of ralstonia solanacearum biovar2

Specific Primers For Identification and Detection of Three Major Desert Truffle Species in Iran with Special Reference to Terfezia claveryi

ABSTRACT- Desert truffles are hypogeous ascomyceteous ectomycorrhizal fungi, occurring in arid and semi-arid ecosystems. A PCR-based method was developed for the identification of 3 major desert truffles of Iran: Terfezia claveryi, Tirmania pinoyi and Tirmania nivea based on internal transcribed spacers of rDNA. Two specific PCR primers were designed for T. claveryi, 4 for T. pinoyi, and 2 for ...

Comparison of general fungal and basidiomycete-specific ITS primers for identification of wood decay fungi

The identity of the fungi associated with and responsible for wood decay could lead to the development of more environmentally benign wood preservative systems. In this study we have reconstructed the phylogenetic tree of a select group of basidiomycete fungi using nucleotide sequences of the internal transcribed spacer (ITS) region. We then compared this to trees generated from restriction fra...

PCR-based identification of Erysiphe pulchra and Phyllactinia guttata from Cornus florida using ITS-specific primers

The internal transcribed spacer (ITS) regions of rDNA and the intervening 5.8S rRNA gene for the powdery mildew fungi Erysiphe (sect. Microsphaera) pulchra and Phyllactinia guttata were amplified using standard polymerase chain reaction (PCR) protocols and the universal primer pairs, ITS1 and ITS4. PCR products for ITS were analysed by electrophoresis in a 1.5% agarose gel and sequenced. The si...

Validation and Development of COI Metabarcoding Primers for Freshwater Macroinvertebrate Bioassessment