One-Step Procedure for Screening Recombinant Plasmids by Size

One-step procedure for screening recombinant plasmids by size.

Rapid screening procedure for detection of plasmids in streptococci.

An enrichment procedure, yielding plasmid deoxyribonucleic acid preparations normally containing less than 5% chromosomal contamination, has been devised for the isolation of plasmids from virtually all species of streptococci. The procedure is rapid, reproducible, and inexpensive, requiring no radioisotopes or density gradient centrifugation. The procedure can be used for routine screening of ...

A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

A procedure for extracting plasmid DNA from bacterial cells is described. The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes. The principle of the method is selective alkaline denaturation of high molecular weight chromosomal DNA while covalently closed circul...

One-step solid-phase extraction procedure for F(2)-isoprostanes.

brovascular occlusive disease, or from peripheral arterial occlusive disease. As shown in Table 1, there were distinctly higher homocysteine concentrations for seropositive subjects in subgroups of age, renal function, smoking status, and MTHFR gene mutations. These variables influencing serum homocysteine, as well as vitamin B 6 , vitamin B 12 , and folic acid, are moreover homogeneously (not ...

Improved Procedure for Screening Expression Libraries for Novel Autoantigens

The standard method for immunoscreening of a cDNA expression library is time-consuming becauseof the production of a large proportion of false positives during the first and second round of screening.This problem is more important when a sensitive chemiluminescence detection system is used. Due tothe high sensitivity of the detection system, there is a need to avoid false posi...