One-step method of phosphatidylcholine extraction and separation

One-step method of phosphatidylcholine extraction and separation.

Rapid One-Step Separation and Purification of Recombinant Phenylalanine Dehydrogenase in Aqueous Two-Phase Systems

Background: Phenylalanine dehydrogenase (PheDH EC 1.4.1.20) is a NAD+-dependent enzyme that performs the reversible oxidative deamination of L-phenylalanine to phenylpyruvate. It plays an important role in detection and screening of phenylketonuria (PKU) diseases and production of chiral intermediates as well. The main goal of this study was to find a simple and rapid alternative method for pur...

A SPECIFIC, SENSITIVE, AND ONE-STEP EXTRACTION RADIOIMMUNOASSAY OF TESTOSTERONE FOR CLINICAL DIAGNOSIS

Testosterone was measured using antibodies raised against testosterone II B-carboxymethyl ether bovine serum albmin (T-IIB-CME-BSA) and testosterone 3- O-carboxymethyl oxime-BSA as immunogen. The antibody produced in this study exhibits minimal cross reactivity with the structurally related steroids specially 5 dihydrotestosterone (5 DHD. This allows to ommit the clean up step and measure ...

A multi-step centrifuge method for extraction of leucocytes and preparation of Tc-HMPAO labeled leucocytes

Background: Labeled leucocytes could be used for localization of infection foci after surgeries or in inflammatory diseases including inflammatory bowel diseases. Extraction of leucocytes needs 10% Hetastarch which is not available in Iran. We provide a method employing multiple centrifuges to extract and label leucocytes with Tc-HMPAO. Methods: The study was conducted from April to June 2018 ...

An Implicit One-Step Method

We have learned that the numerical solution obtained from Euler's method, converges to the exact solution () of the initial value problem ′ = (,), (0) = 0 , as ℎ → 0. We now analyze the convergence of a general one-step method of the form +1 = + ℎΦ(, , ℎ), for some continuous function Φ(, , ℎ). We define the local truncation error of this one-step method by (ℎ) = (+1) − () ℎ − Φ(, (), ℎ). That ...