On chip real time monitoring of B-cells hybridoma secretion of immunoglobulin
نویسندگان
چکیده
منابع مشابه
On chip real time monitoring of B-cells hybridoma secretion of immunoglobulin.
The secretions of molecules by cells are of tremendous interest for both fundamental insights studies and medical purposes. In this study, we propose a new biochip-based approach for the instantaneous monitoring of protein secretions, using antibody production by B lymphocytes cultured in vitro. This was possible thanks to the Surface Plasmon Resonance imaging (SPRi) of a protein biochip where ...
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Effect of Doxorubicin on Mouse Hybridoma B Cells: Stimulation of Immunoglobulin Synthesis and Secretion1
The purpose of these studies was to investigate whether the celldifferentiating effect of anthracyclines can trigger an over-production and secretion of molecules that may interfere with the tumor-host relation ship. We exposed mouse hybridoma B-cells, which are devoted to immunoglobulin production, to doxorubicin (10-40 ng/ml). We found that most doxorubicin-treated cells secreted 3to 5-fold h...
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ژورنال
عنوان ژورنال: Biosensors and Bioelectronics
سال: 2011
ISSN: 0956-5663
DOI: 10.1016/j.bios.2010.09.044