OMIP-003: Phenotypic analysis of human memory B cells

OMIP-020: phenotypic characterization of human γδ T-cells by multicolor flow cytometry.

THIS panel was composed and optimized to investigate the differentiation stages of human cd T-cells in cryopreserved peripheral blood mononuclear cells (PBMC) from healthy individuals (Tables 1 and 2). As the majority of pan-cd T-cell antibodies available commercially proved to be inappropriate for detecting all cd T-cell populations in combination with other markers, this panel provides an ess...

Human IgM+CD27+ B cells: memory B cells or "memory" B cells?

Memory B cells are generated in germinal centers (GC) and contribute to serological immunity by rapidly differentiating into plasma cells. Human memory B cells can be identified by the expression of CD27. These cells exhibit more rapid responses than naive (CD27-) B cells following stimulation in vitro, consistent with the heightened kinetics of secondary responses in vivo. CD27+ B cells expres...

Functional and phenotypic analysis of human memory CD8+ T cells expressing CXCR3.

Several chemokine receptors play an important role in the migration of naïve, memory, and effector T cells. Flow cytometric analyses showed that human CD8+ T cells with naïve (CD27+ CD28+ CD45RA+) or memory (CD27+ CD28+/- CD45RA+) phenotypes included a population expressing a high level of CXC chemokine receptor 3 (CXCR3high) and one expressing a low level of it (CXCR3low), but those with the e...

Phenotypic Analysis of Pneumococcal Polysaccharide-Specific B Cells

Comment on "phenotypic analysis of pneumococcal polysaccharide-specific B cells".

The phenotype of B cells responsible for the production of anti-pneumococcal polysaccharide Ab has been unclear. Although individuals that respond poorly to the 23-valent pneumococcal polysaccharide (PPS) vaccine, Pneumovax, such as children <2 y, the asplenic, and a subset of common variable immunodeficiency patients, are profoundly deficient or lack IgM memory cells (CD27(+)IgM(+)), they are ...