Oligonucleotides as Primers for Polynucleotide Phosphorylase
نویسندگان
چکیده
منابع مشابه
Oligonucleotides as primers for polynucleotide phosphorylase.
Ribonucleoside diphosphates react to form polyribonucleotides and inorganic phosphate. Studies of the nature of nucleotide incorporation into nucleic acid in Escherichia coli led to the recognition of a similar reaction by Littauer and Kornberg (3). Beers (4,5) and Olmsted (6,7) have investigated the phosphorylase in Micrococcus lysodeikticus. Heppel et al. (8-10) have elucidated the structure ...
متن کاملThe dependence of reactions catalyzed by polynucleotide phosphorylase on oligonucleotides.
summarize our knowledge of the mechanism, specificity, and properties of enzyme preparations from several sources, and the purification procedures that have been reported. Previous studies by Singer, Heppel, and Hilmoe (3, 4) led to the suggestion that polynucleotide phosphorylase may be unable to catalyze the initiation of new polyribonucleotide chains, but rather that it acts by the elongatio...
متن کاملFrom polynucleotide phosphorylase to neurobiology.
In the fall of 1944, I enrolled at the Hebrew University of Jerusalem. The student body numbered about 700 and the choice of faculties was somewhat limited.My hopewas to study medicine, but the plans to open a medical school were still at the drawing board stage. I therefore chose to study chemistry with biochemistry and bacteriology as minor subjects, which I thought I would need later if I we...
متن کاملThe effect of chain length on the phosphorolysis of oligonucleotides by polynucleotide phosphorylase.
The kinetics of phosphorolysis of compounds in the series (Ap),A (II = 2 to 8), p(Ap),A (n = 2 to 4), and (Up)I;cT, (II = 3 to 6) has been studied. K, and V,,, values were obtained for all compounds. K, decreases with increasing chain length and reaches a minimum value with compounds containing 5 phosphate residues. With the shorter compounds the dependence of K, on the nucleoside residues is a...
متن کاملPolynucleotide phosphorylase of Micrococcus lysodeiktpcus. III. The apparent arsenolysis of nucleoside diphosphates by polynucleotide phosphorylase.
The enzyme, polynucleotide phosphorylase, catalyzes the polymerization of nucleoside diphosphates to polyribonucleotides with the formation of inorganic orthophosphate (1, 2). The reaction is readily reversible, and the phosphorolysis of polyribonucleotides has been studied extensively (3-6). Several recent reviews (7-9) afford extensive summaries of the literature. In the accompanying paper (l...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1960
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)67932-9