Oligonucleotide arrays: information from replication and spatial structure
نویسندگان
چکیده
منابع مشابه
A normalisation method for oligonucleotide arrays motivated by spatial biases
Many oligonucleotide arrays contain spatial flaws. Standardisation methods such as quantile scaling make no reference to the spatial nature of the flaws. However, the spatial compactness of such flaws can be exploited to provide the basis for corrections that are quite independent of any subsequent chip-wide correction procedure (such as quantile scaling). With three or more replicate arrays, v...
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Affymetrix high-density oligonucleotide array is a tool that has the capacity to simultaneously measure the abundance of thousands of mRNA sequences in biological samples. In order to allow direct array-to-array comparisons, normalization is a necessity. When deciding on an appropriate normalization procedure there are a couple questions that need to be addressed, e.g., on which level should th...
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Oligonucleotide microarrays are based on the hybridization of labeled mRNA molecules to short length oligonucleotide probes on a glass surface. Two effects have been shown to affect the raw data: the sequence dependence of the probe hybridization properties and the chemical saturation resulting from surface adsorption processes. We address both issues simultaneously using a physically motivated...
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ژورنال
عنوان ژورنال: Bioinformatics
سال: 2005
ISSN: 1367-4803,1460-2059
DOI: 10.1093/bioinformatics/bti668