Oligonucleotide Array CGH Analysis of a Robust Whole Genome Amplification Method
نویسندگان
چکیده
منابع مشابه
Oligonucleotide Array CGH Analysis of a Robust Whole Genome Amplification Method
In recent years, array-based Comparative Genomic Hybridization (aCGH) has been refined to determine chromosomal changes at progressively higher resolutions (1). This evolving technology is, however, somewhat hampered by the large DNA input requirement—a minimum of 150,000 copies of a human genome, or 0.5 μg, are generally needed per sample to process one CGH array. GenomePlex® Whole Genome Ampl...
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Genomic instability is a classic hallmark of cancer and genetic disorders. The Agilent Oligonucleotide array-based Comparative Genomic Hybridization (aCGH) platform lets you profile DNA copy number variations on a highthroughput and genome-wide scale. We have developed a rapid, low-input whole genome amplification (WGA) method for aCGH analysis that pairs the Sigma GenomePlex WGA Kit with Agile...
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BACKGROUND Array Comparative Genomic Hybridization (array CGH) is increasingly applied on DNA obtained from formalin-fixed paraffin-embedded (FFPE) tissue, but in a proportion of cases this type of DNA is unsuitable. Due to the high experimental costs of array CGH and unreliable methods for DNA quality testing, better prediction methods are needed. The aim of this study was to accurately determ...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2007
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000112417