Oilbody Proteins in Microspore-Derived Embryos of Brassica napus
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چکیده
منابع مشابه
Triacylglycerol Bioassembly in Microspore-Derived Embryos of Brassica napus L. cv Reston.
Erucic acid (22:1) was chosen as a marker to study triacylglycerol (TAG) biosynthesis in a Brassica napus L. cv Reston microspore-derived (MD) embryo culture system. TAGs accumulating during embryo development exhibited changes in acyl composition similar to those observed in developing zygotic embryos of the same cv, particularly with respect to erucic and eicosenoic acids. However, MD embryos...
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The effect of aqueous smoke extract, derived from Tanacetum parthenium, onin vitro plantlet regeneration from rapeseed (Brassica napus L. cv. Topas)microspore-derived embryos (MDEs) was evaluated in this study. Inoculation ofrapeseed MDEs with the smoke-extract dilutions (1:250, 1:500, 1:1000 and 1:2000)for various times (5, 10 and 15 min) significantly enhanced the percentage ofregenerated pla...
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Storage protein gene expression, characteristic of mid- to late embryogenesis, was investigated in microspore embryos of rapeseed (Brassica napus). These embryos, derived from the immature male gametophyte, accumulate little or no detectable napin or cruciferin mRNA when cultured on hormone-free medium containing 13% sucrose. The addition of abscisic acid (ABA) to the medium results in an incre...
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Exogenous applications of brassinolide (BL) increased the number and quality of microspore-derived embryos (MDEs) whereas treatments with brassinazole (BrZ), a BL biosynthetic inhibitor, had the opposite effect. At the optimal concentration (4x10(-6) M) BrZ decreased both embryo yield and conversion to less than half the value of control embryos. Metabolic studies revealed that BL levels had pr...
متن کاملFatty acid breakdown in developing embryos of Brassica napus L.
Developing Brassica napus embryos are primarily concerned with the accumulation of storage products, namely oil, starch and protein. The presence of fatty acid catabolic pathways in the background of this biosynthetic activity was investigated. Enzymes involved in the process of lipid mobilization, such as malate synthase and isocitrate lyase, are detectable towards the late stages of embryo de...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1991
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.97.3.1051