O-039 Fertilization: Mechanisms of pronuclear migration and genome unification

Abstract Most early human embryos contain aneuploid cells. Aneuploidy often arises during the mitotic divisions of embryo, but its origin remains elusive. Human zygotes that cluster their nucleoli at pronuclear interface are thought to be more likely develop into healthy euploid embryos. I will present data showing how parental genomes with in each pronucleus and bovine zygotes. Parental genome clustering is required for reliable unification after fertilization. During migration intact pronuclei, polarize toward other a process driven by centrosomes, dynein, microtubules, nuclear pore complexes. The maternal paternal chromosomes eventually interface, close proximity other, yet separated. ensures rapid upon envelope breakdown. However, fails, leading chromosome segregation errors micronuclei incompatible embryonic development. In summary, our reveal why nucleolar correlates formation addition, we used automated analysis trajectories clustering. Our results support use as parameter identify higher developmental potential.