Nutritional Variation of Human Tubercle Bacilli Growth on Glycerol-free Media
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Factors Affecting the Growth of Tubercle Bacilli in Liquid Media
1. Certain water-soluble esters of long chain fatty acids (in particular of oleic acid) favor submerged and diffuse growth of mycobacteria throughout the depth of synthetic liquid media. 2. Esters of oleic acid increase considerably the amount of growth yielded by avian strains in synthetic media. 3. The addition of serum albumin to synthetic liquid media permits visible growth of minimal inocu...
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It has been difficult to cultivate human tubercle bacilli in agar medium unless bovine serum, or a fraction therefrom, was added aseptically, since the factor present in the serum is thermolabile (Dubos and Middlebrook, 1947). On the other hand, egg yolk or whole egg media may be used, but the difficulties in preparing the media render the methods uncertain and cumbersome. Hence, a thermostable...
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All preparations of sphingomyelin tested, whatever the tissues from which they originated, were found to enhance the growth of tubercle bacilli in vitro. Cerebrosides were inactive in this respect. Sphingomyelin promotes growth through two independent mechanisms: (a) It neutralizes the toxicity of long chain fatty acids probably by forming with them inert complexes. This protective effect facil...
متن کاملThe chemistry of the lipides of tubercle bacilli; concerning inositol glycerol diphosphoric acid, a component of the phosphatide of human tubercle bacilli.
In studies conducted in this laboratory dealing with the composition of the phosphatide isolated from the human tubercle bacillus, strain H-37, it was recognized early that an organic phosphoric acid of the formula C9H20014P2 could be separated in the form of the barium salt from the water-soluble cleavage products after the phosphatide had been saponified with alcoholic potassium hydroxide (I)...
متن کاملSelective media in the isolation of tubercle bacilli from tissues.
Direct culture on Lowenstein-Jensen slopes and on three media made selective for tubercle bacilli by the addition of four antibacterial agents was compared with guinea-pig inoculation on 490 tissue specimens. Tubercle bacilli were obtained from 15 specimens by culture and 14 by guinea-pig inoculation; only one specimen was positive by guinea-pig and not by culture. The most efficient culture me...
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ژورنال
عنوان ژورنال: Nippon Saikingaku Zasshi
سال: 1964
ISSN: 1882-4110,0021-4930
DOI: 10.3412/jsb.19.197