Nucleotide Sequence of a Pumpkin Phloem Lectin cDNA

Nucleotide sequence of a pumpkin phloem lectin cDNA.

One of the characteristic events that occurs during phloem differentiation in dicotyledonous plants is the appearance of phloem protein within the sieve elements and companion cells of the phloem tissue (Esau and Cronshaw, 1967). Two very abundant phloem proteins, PPl and PP2, have been isolated from phloem exudates of pumpkin (Cucurbita maxima) (Read and Northcote, 1983). PPl is a 96-kD struct...

Pumpkin phloem lectin genes are specifically expressed in companion cells.

Pumpkin phloem exudate contains two abundant phloem proteins: PP1 is a 96-kD protein that forms polymeric filaments in vivo, and PP2 is a 48-kD dimeric lectin. Polyclonal antibodies raised against pumpkin phloem exudate were used to isolate several cDNAs corresponding to PP1 and PP2. RNA gel blot analysis indicated that PP1 is encoded by an mRNA of approximately 2500 nucleotides, whereas PP2 su...

Nucleotide sequence of cDNA encoding for preprochymosin in native goat (Capra hircus) from Iran

Prochymosin is one of the most important aspartic proteinases used as a milk-clotting enzyme in cheese production. In the present investigation we report sequence of cDNA encoding goat ( Capra hircus ) preprochymosin and compare its nucleotide and deduced amino acid sequences with sequences of other ruminants preprochymosin. As bovine prochymosin, the caprine prochymosin cDNA encodes 365 amino ...

Rapid affinity-purification and physicochemical characterization of pumpkin (Cucurbita maxima) phloem exudate lectin.

The chito-oligosaccharide-specific lectin from pumpkin (Cucurbita maxima) phloem exudate has been purified to homogeneity by affinity chromatography on chitin. After SDS/PAGE in the presence of 2-mercaptoethanol, the pumpkin phloem lectin yielded a single band corresponding to a molecular mass of 23.7 kDa, whereas ESI-MS (electrospray ionization MS) gave the molecular masses of the subunit as 2...

Rapid affinity-purification and physico- chemical characterization of pumpkin (Cucurbita maxima) phloem exudate lectin