Nucleotide sequence at the 5' terminus of adenovirus 2 late messenger RNA.
نویسندگان
چکیده
منابع مشابه
Nucleotide sequence at the junction between the coding region of the adenovirus 2 hexon messenger RNA and its leader sequence.
We have determined a 139-base-pair sequence of adenovirus 2 DNA that is located immediately leftwards of the cleavage site for endonuclease Sma I at position 51.1. The established sequence includes the hexon AUG initiator codon, located 75--77 nucleotides leftwards of this cleavage site, and codons for the first 26 amino acids of the hexon polypeptide. By the use of purified hexon mRNA as a tem...
متن کاملSpliced segments at the 5 ' terminus of adenovirus 2 late mRNA * ( adenovirus 2 mRNA processing / 5 ' tails on mRNAs /
An mRNA fraction coding for hexon polypeptide, the major virion structural protein, was purified by gel electrophoresis from extracts of adenovirus 2-infected cells late in the lytic cycle. The mRNA sequences in this fraction were mapped between 51.7 and 61.3 units on the genome by visualizing RNA-DNA hybrids in the electron microscope. When hybrids of hexon mRNA and single-stranded restriction...
متن کاملSequence of methylated nucleotides at the 5'-terminus of adenovirus-specific RNA.
RNA labeled with [methyl-3H] methionine and [14C]uridine was isolated from the cytoplasm of adenovirus-infected cells and purified by poly(U)-Sepharose chromatography and hybridization to filters containing immobilized adeovirus DNA. Analysis by dimethyl sulfoxide-sucrose gradient sedimentation suggested that the major mRNA species were methylated. 7-Methylguanosine was identified at the 5'-ter...
متن کاملThe nucleotide sequence at the 5'-terminus of the Q RNA minus trand.
The sequence of the first 52 nucleotides at the 5'-end of the Qbeta minus strand has been determined and found to be complementary in an antiparallel fashion to the 3'-terminal region of the Qbeta plus strand. There are few similarities between the corresponding sequences of Qbeta plus and minus strands; however, both have a hydrogen-bonded loop close to the 5'-end. The sequence of the 3'-termi...
متن کاملSpliced segments at the 5' terminus of adenovirus 2 late mRNA. 1977.
An mRNA fraction coding for hexon polypeptide, the major virion structural protein, was purified by gel electrophoresis from extracts of adenovirus 2-infected cells late in the lytic cycle. The mRNA sequences in this fraction were mapped between 51.7 and 61.3 units on the genome by visualizing RNA-DNA hybrids in the electron microscope. When hybrids of hexon mRNA and single-stranded restriction...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1979
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)37843-2