Nucleoprotein Complexes in Simian Virus 40-Infected Cells
نویسندگان
چکیده
منابع مشابه
Nucleoprotein complexes in simian virus 40-infected cells.
When African green monkey kidney cells (BSC-1) were infected with simian virus 40 (SV40) and extracted with 0.25% Triton X-100 after exposure to (3)H-thymidine, the (3)H-SV40 deoxyribonucleic acid (DNA) was present in a form which had a sedimentation coefficient in sucrose gradients of 44S. The change from the sedimentation coefficient of purified SV40 DNA (21S) was shown to result from the ass...
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Viral nucleoprotein complexes were extracted from the nuclei of simian virus 40 (SV40)-infected TC7 cells by low-salt treatment in the absence of detergent, followed by sedimentation on neutral sucrose gradients. Two forms of SV40 nucleoprotein complexes, those containing SV40 replicative intermediate DNA and those containing SV40 (I) DNA, were separated from one another and were found to have ...
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The complete nucleotide sequence has been determined for three newly cloned evolutionary variants from two different independently generated evolutionary series ( 1 100 and 2100 series) of simian virus 4 0 (SV40). These naturally arising variants, designated ev-1110, ev-2102, and eu-2114, were isolated after five high multiplicity serial passages. The structure of the variants consists of a mon...
متن کاملRecombination in Simian Virus 40-infected Cells
The complete nucleotide sequence has been determined for three newly cloned evolutionary variants from two different independently generated evolutionary series ( 1 100 and 2100 series) of simian virus 4 0 (SV40). These naturally arising variants, designated ev-1110, ev-2102, and eu-2114, were isolated after five high multiplicity serial passages. The structure of the variants consists of a mon...
متن کاملNew way to isolate simian virus 40 nucleoprotein complexes from infected cells: use of a thiol-specific reagent.
A new method for the isolation of simian virus 40 nucleoprotein complexes from nuclei of lytically infected cells is described. The method is based on the addition of a thiol-specific reagent, 5'5'-dithiobis(2-nitrobenzoic acid), to lysis and extraction buffers. By inhibiting an uncoating activity during simian virus 40 extraction, 5'5'-dithiobis (2-nitrobenzoic acid) allows the use of efficien...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1971
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.8.4.363-371.1971