منابع مشابه
Advances in Nucleic Acid Detection and Quantification
The last decade has seen many changes in molecular biology at the bench, as we have moved away from a primary goal of cataloguing genes andmRNA towards techniques that detect and quantify nucleic acid molecules even within single cells. With the invention of the polymerase chain reaction (PCR), a nucleic acid sequence could now be amplified to generate a large number of identical copies, and th...
متن کاملFluorescent hybridization probes for nucleic acid detection.
Due to their high sensitivity and selectivity, minimum interference with living biological systems, and ease of design and synthesis, fluorescent hybridization probes have been widely used to detect nucleic acids both in vivo and in vitro. Molecular beacons (MBs) and binary probes (BPs) are two very important hybridization probes that are designed based on well-established photophysical princip...
متن کاملNucleic acid detection with CRISPR-Cas13a/C2c2.
Rapid, inexpensive, and sensitive nucleic acid detection may aid point-of-care pathogen detection, genotyping, and disease monitoring. The RNA-guided, RNA-targeting clustered regularly interspaced short palindromic repeats (CRISPR) effector Cas13a (previously known as C2c2) exhibits a "collateral effect" of promiscuous ribonuclease activity upon target recognition. We combine the collateral eff...
متن کاملDeveloping nucleic acid-based electrical detection systems
Development of nucleic acid-based detection systems is the main focus of many research groups and high technology companies. The enormous work done in this field is particularly due to the broad versatility and variety of these sensing devices. From optical to electrical systems, from label-dependent to label-free approaches, from single to multi-analyte and array formats, this wide range of po...
متن کاملPolyaniline nanofibres for fluorescent nucleic acid detection.
In this communication, we demonstrate for the first time that conducting polymer polyaniline (PANI) nanofibres can serve as a novel fluorescent sensing platform for nucleic acid detection with a high selectivity down to single-base mismatch.
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ژورنال
عنوان ژورنال: Chemistry & Biology
سال: 2010
ISSN: 1074-5521
DOI: 10.1016/j.chembiol.2010.02.003