Nucleic acid detection using G-quadruplex amplification methodologies
نویسندگان
چکیده
منابع مشابه
Amplification of G-quadruplex DNAzymes using PCR-like temperature cycles for specific nucleic acid and single nucleotide polymorphism detection.
A nucleic acid sensor, based on the amplified formation of G-quadruplex DNAzymes by polymerase chain reaction (PCR)-like temperature cycles, was developed. This "turn-on" process allowed effective detection of specific nucleic acid targets and identification of single nucleotide polymorphisms (SNPs).
متن کاملG-Quadruplex Nucleic Acids
1 IRB Barcelona, IQAC-CSIC, Jordi Girona 16-26, 08034 Barcelona, Spain 2 2 INSERM U869, Laboratoire ARNA, Université de Bordeaux 2, Institut Européen de Chimie-Biologie, 2 rue Robert Escarpit, 33607 Pessac Cedex, France 3Dipartimento di Chimica Organica e Biochimica, Università degli Studi di Napoli “Federico II”, I-80126 Naples, Italy 4 Faculty of Mechanical Engineering, University of Maribor,...
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A protein-free, isothermal, self-amplified nucleic acid sensing system which was a G-quadruplex integrated hybridization chain reaction (GQ-HCR) system was developed. The G-quadruplex was closed two-thirds in the loop and one-third in the stem of one of the GQ-HCR hairpin probes. In the absence of the target molecule, the GQ-HCR probes stayed as inactive meta-stable hairpin structures and the G...
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The split G-quadruplex DNAzyme has emerged as a valuable tool for visual DNA detection. Here, we successfully integrated colorimetric split G-quadruplex DNAzyme assay with nucleic acid sequence-based amplification to generate a novel detection approach, allowing visual and rapid detection for the RNA of Shimen and HCLV strains of Classic Swine Fever Virus (CSFV). CSFV is a RNA virus that causes...
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We report here the development of a rapid PCR microfluidic system comprising a double-shaft turntable and centrifugal-based disc that rapidly drives the PCR mixture between chambers set at different temperatures, and the bidirectional flow improved the space utilization of the disc. Three heating resistors and thermistors maintained uniform, specific temperatures for the denaturation, annealing,...
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ژورنال
عنوان ژورنال: Methods
سال: 2013
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2013.10.003