Nuclear Location of the 16K Non-structural Protein of Tobacco Rattle Virus
نویسندگان
چکیده
منابع مشابه
Functional characterization and subcellular localization of the 16K cysteine-rich suppressor of gene silencing protein of tobacco rattle virus.
The 16 kDa cysteine-rich protein (16K) of tobacco rattle virus (TRV) is known to partially suppress RNA silencing in Drosophila cells. In this study, we show that 16K suppresses RNA silencing in green fluorescent protein (GFP)-transgenic Nicotiana benthamiana plants using an Agrobacterium-mediated transient assay. 16K slightly reduced the accumulation of short interfering RNAs (siRNA) of GFP, s...
متن کاملThe complete nucleotide sequence of tobacco rattle virus RNA-1.
The complete nucleotide sequence of tobacco rattle virus (TRV) strain SYM RNA-1 was determined from a series of overlapping cDNA clones. cDNA prepared by primer extension was used to determine the exact 5' terminus. The RNA sequence was 6791 nucleotides in length and contained four open reading frames (ORFs). The ORF nearest the 5' terminus coded for a polypeptide of predicted mol. wt. 134,000 ...
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Inactivation and mutagenesis of tobacco rattle virus by nitrous acid.
The rate of inactivation of tobacco rattle virus RNA by o.z M-nitrous acid at pH 4"o was comparable to that of tobacco mosaic virus RNA. In o'o025 M-nitrous acid at pH 6.0 this rate was greatly diminished, and was slower than that of intact virus. Possible reasons for this are discussed. Methods are described for the induction of mutants of tobacco rattle virus by nitrous acid, and for the sele...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1991
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-72-8-1811