Nuclear Cytidine 5'-Monophosphosialic Acid Synthetase
نویسندگان
چکیده
منابع مشابه
The sialic acids. X. Purification and properties of cytidine 5'-monophosphosialic acid synthetase.
An enzyme, cytidine monophosphate sialic acid synthetase, was purified from hog submaxillary glands. This enzyme (or enzymes) catalyzes the synthesis of CMP-N-acetylneuraminic acid or CMP-N-glycolylneuraminic acid or both and inorganic pyrophosphate from CTP and the corresponding sialic acids. The preparation was inactive with other known sialic acids and analogues of these compounds, and was a...
متن کاملMammalian cytidine 5'-monophosphate N-acetylneuraminic acid synthetase: a nuclear protein with evolutionarily conserved structural motifs.
Sialic acids of cell surface glycoproteins and glycolipids play a pivotal role in the structure and function of animal tissues. The pattern of cell surface sialylation is species- and tissue-specific, is highly regulated during embryonic development, and changes with stages of differentiation. A prerequisite for the synthesis of sialylated glycoconjugates is the activated sugar-nucleotide cytid...
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Calf liver CTP synthetase was purified 2000-fold from cytosol. The formation of 1 mol of CTP from UTP was accompanied by the cleavage of 1 mol of ATP to ADP and the formation of 1 mol of L-glutamate from L-glutamine. The stoichiometry of the liver enzyme reaction was identical with that found for the Escherichia coli B enzyme (Levitzki, A., and Koshland, D. E., Jr. (1971) Biochemistry 10, 3365-...
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Cytidine triphosphate synthetase (CTP synthetase) activity was measured in extracts from normal and malignant lymphoid cells. A range of enzyme activities was found in the majority of the lymphoproliferative disorders examined, with acute lymphocytic leukemia, nodular poorly differentiated lymphocytic lymphoma, and diffuse histiocytic (large cell) lymphoma (DHL) types exhibiting the widest rang...
متن کاملEnzymatic synthesis of cytidine 5'-monophospho-N-acetylneuraminic acid.
We have established an efficient method for enzymatic production of cytidine 5'-monophospho-N-acetylneuraminic acid (CMP-NeuAc) from inexpensive materials, N-acetylglucosamine (GlcNAc) and cytidine 5'-monophosphate (CMP). The Haemophilus influenzae nanE gene encoding GlcNAc 6-phosphate (GlcNAc 6-P) 2-epimerase and the Campylobacter jejuni neuB1 gene encoding N-acetylneuraminic acid (NeuAc) synt...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1970
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)63152-7