Novel urine-based DNA methylation biomarkers for urothelial bladder carcinoma detection in patients with hematuria
نویسندگان
چکیده
Background Urothelial bladder carcinoma (UBC) is usually detected during work-up for hematuria. Cystoscopy and/or contrast-enhanced imaging are the gold standard tools UBC diagnosis, despite limited by being invasive, expensive and low yield in small flat tumors.Objectives To assess diagnostic performance of urine-based DNA methylation six genes (GATA4, P16, P14, APC, CDH1 CD99) detection patients with hematuria.Patients methods Voided urine was collected from consecutive presented hematuria cytology assay assigned using methylation-specific Polymerase Chain Reaction (PCR). Further assessment office cystoscopy subsequent inpatient cystoscopic biopsy positive findings done. The characteristics were assessed based on its capability to predict UBC.Results We included 246 study identified macroscopic 204 (82.9%) patients. Positive found 78 (31.7%) GATA4, CD99 127 (51.6%), 52 (21.1%), 117 (47.6%), 106 (43.1%), 90 (36.6%) 71 (28.9%) patients, respectively. sensitivity ranges 35% (95%CI: 31–39) 83% 79–87). Optimal specificity (SP) (100%) noted APC genes. While other P14 CD99), SP 95% 92–98), 96% 92–99) 97% 93–99), On multivariate logistic regression analysis, all exclusively demonstrated independent prediction UBC. receiver operator characteristic (ROC) tested showed superior area under curve (AUC) when compared cytology.Conclusions have developed a novel predictive capacity over cytology.
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ژورنال
عنوان ژورنال: Arab journal of urology
سال: 2023
ISSN: ['2090-598X', '2090-5998']
DOI: https://doi.org/10.1080/2090598x.2023.2208492