Novel One-Step Single-Tube Nested Quantitative Real-Time PCR Assay for Highly Sensitive Detection of SARS-CoV-2
نویسندگان
چکیده
منابع مشابه
A sensitive and quantitative single-tube real-time reverse transcriptase-PCR for detection of enteroviral RNA.
BACKGROUND Enteroviruses (EVs) are significant human pathogens. Rapid and sensitive diagnostic techniques are desirable. OBJECTIVES To develop a quantitative single-tube real-time reverse transcription-polymerase chain reaction (RT-PCR) for human enterovirus ribonucleic acid (RNA) (QPCR), with protection against amplimer contamination. STUDY DESIGN The method was evaluated with serial dilut...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2020
ISSN: 0003-2700,1520-6882
DOI: 10.1021/acs.analchem.0c01884