Novel Observations of Peroxiredoxin-2 Profile and Protein Oxidation in Skeletal Muscle From Pigs of Differing Residual Feed Intake and Health Status

This study’s objective was to determine the impact of a dual respiratory and enteric bacterial health challenge on antioxidant protein peroxiredoxin-2 (Prdx-2) profile oxidation in skeletal muscle pigs from 2 lines that were divergently selected for residual feed intake (RFI). The hypotheses (1) differences exist Prdx-2 between RFI infection status (2) less efficient high-RFI health-challenged have greater cellular oxidation. Barrows (50 ± 7 kg, N = 24) 11th generation (n 12) low-RFI Iowa State University used. Pigs 6 per line) inoculated with Mycoplasma hyopneumoniae Lawsonia intracellularis (MhLI) day 0 post induce challenge. Uninoculated served as controls line). Necropsy at 21 d infection. Sarcoplasmic oxidation, various forms Prdx-2, glyceraldehyde 3-phosphate dehydrogenase (GAPDH) content determined. Neither line nor significantly affected carbonylation. Under nonreducing conditions, MhLI had amount slower-migrating GAPDH band (P 0.017), indicating oxidative modification. Regardless status, total 0.035), decamer 0.0007), higher ratio hyperoxidized peroxiredoxin relative 0.028) than pigs. increased pool active suggests stress high- versus increase oxidized seen pigs—particularly pigs—may be response MhLI. work proteins are important growth health-challenge situations.