Normalization of Luciferase Reporter Assays under Conditions that Alter Internal Controls

Normalization of luciferase reporter assays under conditions that alter internal controls.

Reporter Assays and More: Applications of NanoLuc® Luciferase

NanoLuc® Luciferase brings exciting new possibilities and improvements to luminescence applications, including protein stability monitoring, detection of protein interactions using BRET, and in vivo imaging. This article highlights several recent papers that illustrate the use of NanoLuc® Technology as a sensitive reporter for challenging applications beyond those of classic bioluminescence rep...

Accelerating the pace of luciferase reporter gene assays.

Luciferase reporter gene assays have gained more importance because of their easy readout, high sensitivity and lack of environmental waste disposal problems. However, several obstacles remain that have prohibited a wider use and the implementation of this type of assay in high-throughput screening programs: (i) Measurements need to be carried out within an active enzyme reaction, and the asses...

Improved Dual-Luciferase Reporter Assays for Nuclear Receptors

Nuclear receptors play important roles in many cellular functions through control of gene transcription. It is also a large target class for drug discovery. Luciferase reporter assays are frequently used to study nuclear receptor function because of their wide dynamic range, low endogenous activity, and ease of use. Recent improvements of luciferase genes and vectors have further enhanced their...

Using Luciferase Reporter Assays to Screen for Gpcr Modulators

GPCR signaling pathways can be categorized into three classes based on the G protein α-subunit involved, Gs, Gq, and Gi/o. These subunits mediate signaling through distinct pathways that lead to the modulation of gene transcription via specific response elements (RE), which are located upstream of the regulated genes. Reporter assays have been used successfully in the process of GPCR-targeted d...