Nonreactivity of American avian group A rotaviruses with subgroup-specific monoclonal antibodies

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Nonreactivity of American avian group A rotaviruses with subgroup-specific monoclonal antibodies.

Nine group A rotavirus isolates recovered from commercially reared poultry in the United States did not react with subgroup 1 (255/60) or subgroup 2 (631/9) specific monoclonal antibodies in an enzyme-linked immunosorbent assay.

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Hybridomas secreting monoclonal antibodies (MAbs) to a group A rotavirus (GAR) were developed by fusion of myeloma cell (SP2/0) with spleen cells of BALB/c mice immunized with semi-purified bovine rotavirus (CR129, G10P11). Two of these rotavirus-specific monoclonal antibodies were further characterized by immunoblotting and also tested for their reactivity with rotaviruses of bovine, equine an...

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Monoclonal Antibodies

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1989

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.27.12.2846-2848.1989