Nonlinear Absorption Microscopy

Nonlinear absorption microscopy.

For the past two decades, nonlinear microscopy has been developed to overcome the scattering problem in thick tissue imaging. Owing to its increased imaging depth and high spatial resolution, nonlinear microscopy becomes the first choice for imaging living tissues. The use of nonlinear optical effects not only facilitates the signal originating from an extremely small volume defined by light fo...

Nonlinear dark-field microscopy.

Dark-field microscopy is a background-free imaging method that provides high sensitivity and a large signal-to-noise ratio. It finds application in nanoscale detection, biophysics and biosensing, particle tracking, single molecule spectroscopy, X-ray imaging, and failure analysis of materials. In dark-field microscopy, the unscattered light path is typically excluded from the angular range of s...

Nonlinear absorption in discrete systems

In the context of nonlinear scattering, a continuous wave incident onto a nonlinear discrete molecular chain of coupled oscillators can be partially absorbed as a result of a 3-wave resonant interaction that couples two HF-waves of frequencies close to the edge of the Brillouin zone. Hence both nonlinearity and discreteness are necessary for generating this new absorption process which manisfes...

Nonlinear Absorption by Porphyrin Supramolecules

High-throughput nonlinear optical microscopy.

High-resolution microscopy methods based on different nonlinear optical (NLO) contrast mechanisms are finding numerous applications in biology and medicine. While the basic implementations of these microscopy methods are relatively mature, an important direction of continuing technological innovation lies in improving the throughput of these systems. Throughput improvement is expected to be imp...