Non-radioisotopic and Semi-quantitative Procedure for Terminal Repeat Amplification Protocol

Real-time quantitative telomeric repeat amplification protocol assay for the detection of telomerase activity.

BACKGROUND Telomerase is a ribonucleoprotein enzyme associated with immortalization and transformation of human cells. The telomeric repeat amplification protocol (TRAP) is widely used for the detection of telomerase activity. The TRAP method, although highly sensitive and specific because it includes PCR amplification, is laborious and does not provide precise quantitative information. METHO...

Simplified method for the telomeric repeat amplification protocol (TRAP).

Since it was first described by Kim et al. in 1994 (4), the telomeric repeat amplification protocol (TRAP) has been used extensively to detect telomerase activity in a variety of tissues. It is a highly sensitive polymerase chain reaction (PCR)-based assay that has been used to detect telomerase activity in a high proportion of cancers of various origins as well as low levels of activity in cer...

Minigel Procedure for Telomeric Repeat Ampli - fication Protocol

Telomeres, the ends of chromosomes, are composed of many kilobases of TTAGGG repeats in vertebrates. These repeats are added by the enzyme telomerase, a ribonucleoprotein DNA polymerase that synthesizes the repeats based on its RNA template. A sensitive assay combining telomerase activity and the polymerase chain reaction (PCR) known as the telomeric repeat amplification protocol (TRAP) has bee...

Dual-color real-time telomeric repeat amplification protocol.

Rapid radioisotopic procedure for determination of nortriptyline in plasma.

With the widespread use of tricyclic antidepressant drugs, the relationship between the concentration of the drug in the plasma and the therapeutic response is of considerable interest. We describe a double-isotope derivative dilution procedure for measuring plasma nortriptyline. In the method, [14C]nortriptyline is used for estimating procedural losses and [3H]acetic anhydride for derivative f...