منابع مشابه
Involvement of GroEL in nif gene regulation and nitrogenase assembly.
Several approaches were used to study the role of GroEL, the prototype chaperonin, in the nitrogen fixation (nif) system. An Escherichia coli groEL mutant transformed with the Klebsiella pneumoniae nif gene cluster accumulated very low to nondetectable levels of nitrogenase components compared with the isogenic wild-type strain or the mutant cotransformed with the wild-type groE operon. In K. p...
متن کاملNitrogenase Complex
The nitrogenase complex carries out the fixation of nitrogen by reducing molecular dinitrogen (N2) to ammonium (NH4 1 ) and also reduces acetylene to ethylene. The two component proteins are dinitrogenase and dinitrogenase reductase. Although different metals can form the metal clusters contained in nitrogenase proteins, all nitrogenases have similar properties. The nitrogenase enzyme system is...
متن کاملThe chaperone GroEL is required for the final assembly of the molybdenum-iron protein of nitrogenase.
It is known that an E146D site-directed variant of the Azotobacter vinelandii iron protein (Fe protein) is specifically defective in its ability to participate in iron-molybdenum cofactor (FeMoco) insertion. Molybdenum-iron protein (MoFe protein) from the strain expressing the E146D Fe protein is partially ( approximately 45%) FeMoco deficient. The "free" FeMoco that is not inserted accumulates...
متن کاملIron-sulfur cluster assembly: NifU-directed activation of the nitrogenase Fe protein.
The NifU protein is a homodimer that is proposed to provide a molecular scaffold for the assembly of [Fe-S] clusters uniquely destined for the maturation of the nitrogenase catalytic components. There are three domains contained within NifU, with the N-terminal domain exhibiting a high degree of primary sequence similarity to a related family of [Fe-S] cluster biosynthetic scaffolds designated ...
متن کاملFeedback inhibition of nitrogenase.
No inhibition of nitrogenase activity by physiological levels of NH4+ or carbamyl phosphate was observed in extracts of Azotobacter vinelandii. All of the 15N2 reduced by cultures which received no NH4+ was found in the cells. By contrast, more than 95% of the 15N2 reduced by cultures which had been given NH4+ was found in the medium. Failure to examine the culture medium would lead to the erro...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Bioenergetics
سال: 2013
ISSN: 0005-2728
DOI: 10.1016/j.bbabio.2012.12.001